金沙990线路检测(中国百科)有限公司-Gaming Group

教育经历:

2008-2013，博士，生物化学与分子生物学，武汉大学
2009-2012，联合培养博士，加州大学圣地亚哥分校
2004-2008，本科，生物技术，武汉大学

工作经历:

2013-2016，博士后，麻省理工学院白头生物医学研究所
2016-现在，研究员，金沙990线路检测(中国百科)有限公司-Gaming Group
2016-现在，研究员，北大-清华生命科学联合中心

荣誉奖励:

2013, 吴瑞奖学金
2013, 湖北省优秀博士论文
2017, 億方学者
2017-2022, 国家重点研发计划专项青年项目，首席科学家

学术任职:

2023-，中国细胞生物学会染色质生物学会委员
2023-，中国遗传学会三维基因组学会委员

杂志任职:

2024-，Genome Biology, 编委（Editorial Board Member）

执教课程:

近代分子生物学史话
细胞核的结构与功能

      研究兴趣包括RNA聚合酶相关的基因表达调控机理与计算理论。以早期胚胎干细胞和癌症细胞为模型，运用基因组学、蛋白质组学、生物信息学、计算机模拟、CRISPR基因编辑、光学成像和生物化学等多学科技术开展基因表达调控原理研究。主要致力于：
1. RNA聚合酶相关的分子机理、疾病与治疗
2. 生命分子计算

2025：
Science Advances | 季雄团队提出FeaSion策略揭示RNA聚合酶磷酸化的特征调控与功能
/homes/Index/news_cont/22/18600.html

NAR | 季雄团队揭示INO80/SWR复合物通过BRD2和染色质景观调控Pol II转录起始
/homes/Index/news_cont/22/17984.html

Nat Commun | 季雄团队揭示RNA聚合酶亚基RPB7偶联磷酸酶CTDP1稳定Pol II并介导转录再起始
/homes/Index/news_cont/22/17632.html

2024：
NAR | 季雄团队揭示RNA聚合酶II降解导致GPCR配体结合基因上调机制
/homes/Index/news_cont/22/17159.html

2023：
Cell Reports | 季雄团队揭示亚细胞定位对RNA聚合酶 III命运调控的决定性作用
/homes/Index/news_cont/22/16683.html

NAR+Transcription+STAR Protoc | 季雄团队在基因表达调控领域取得进展
/homes/Index/news_cont/22/16591.html

Mol Cell | 季雄团队揭示RNA聚合酶II亚基的非经典调控功能
/homes/Index/news_cont/22/16436.html

Cell | 季雄组与陈鹏组合作实现活细胞中染色质修饰的编码表达与串联解析
/homes/Index/news_cont/22/16427.html

2022：
Genome Biology | 季雄团队揭示RNA聚合酶交叉调控关系和机制
/homes/Index/news_cont/22/16351.html

Nature Communications | 季雄团队鉴定RNA聚合酶II转录工厂控制因子RUVBL2
/homes/Index/news_cont/22/16272.html

Mol Cell+iScience | 季雄团队揭示RNA聚合酶II亚基异质性和CTCF绝缘机理
/homes/Index/news_cont/22/16258.html

CELL MOL LIFE SCI | 季雄课题组揭示BRD2介导RNA聚合酶II转录的功能机理
/homes/Index/news_cont/22/16167.html

2020：
Genome Biology & Methods | 季雄课题组在RNA聚合酶与染色质三维结构研究中取得进展
/homes/Index/news_cont/22/15025.html

1. Zhu J, Bao L, Xu S, Ji X*. FeaSion decodes the regulatory landscape and functional diversity of RNA polymerase II CTD phosphorylation. Science Advances. 2025 Nov 28;11(48): eadz2345.
2. Shao C, Hao Y, Jiang L, Wang D, Wang R, Li Y, Wang H, Ge Y, Bai R, Du X, Chen X, Wu T, Gou LT, Wan R, Xu Y, Ji X, Fu XD. Dynamic U2AF cycling defines two phases of cotranscriptional pre-mRNA splicing. Science. 2025 Sep 25;389(6767): eadj9141.
3. Liu M, Xu Q, Wang H*, Ji X*. INO80/SWR remodelers regulate Pol II transcription through BRD2 and chromatin landscape. Nucleic Acids Res. 2025 Sep 5;53(17): gkaf892.
4. Zheng H, Xu Q, Ji D, Yang B, Ji X*. CTDP1 and RPB7 stabilize Pol II and permit reinitiation. Nature Communications. 2025 Mar 4;16(1): 2161.
5. Bao L, Zhu J, Shi T, Jiang Y, Li B, Huang J*, Ji X*., Increased transcriptional elongation and RNA stability of GPCR ligand binding genes unveiled via RNA polymerase II degradation. Nucleic Acids Res. 2024 Aug 12;52(14):8165-8183.
6. Liu Y, Zhangding Z, Liu X, Gan T, Ai C, Wu J, Liang H, Chen M, Guo Y, Lu R, Jiang Y, Ji X., Gao N, Kong D, Li Q, Hu J.*, Fork coupling directs DNA replication elongation and termination. Science. 2024 Mar 15;383(6688):1215-1222.
7. Huang, J.*, Ji, X.*, Never a dull enzyme, RNA polymerase II. Transcription. 2023 Nov;14(1-2):49-67.
8. Tian K, Wang R, Huang J, Wang H, Ji, X.*, Subcellular localization shapes the fate of RNA polymerase III. Cell Reports. 2023 Aug 29;42(8):112941.
9. Wang, R., Xu, Q., Wang, C., Tian, K., Wang, H., Ji, X.*. Multiomic analysis of Cohesin reveals that ZBTB transcription factors contribute to chromatin interactions. Nucleic Acids Res. 2023 Jul 21;51(13):6784-6805.
10. Huang J. *, Bao L., Zhu J., Ji, X.*, Protocol for quantitative analysis of RNA 3'-end processing induced by disassociated subunits using chromatin-associated RNA-seq data. STAR protocol. 2023 Jun 15;4(3):102356.
11. Li, Y., Huang, J., Bao, L., Zhu, J., Duan, W., Zheng, H., Wang, H., Jiang, Y., Liu, W., Zhang, M., Yu, Y., Yi, C., Ji, X.*, Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits. Molecular Cell. 2023 Apr 20;83(8):1280-1297.
12. Ji X., Huang J., Zhu J., Duan W., Li Y., Bao L., Meet the authors: The Ji lab. Molecular Cell. 2023 Apr 20;83(8):1197-1199.
13. Wang, H.*, Zhou R., Ji, X.*, Droplet formation assay for investigating phase-separation mechanisms of RNA Pol II transcription and CTCF functioning. STAR protocol. 2023 Mar 28;4(2).
14. Qin, F.*, Li, B., Wang, H., Ma, S., Li, J., Liu, S., Kong, L., Zheng, H., Zhu, R., Han, Y., Yang, M., Li, K., Ji, X.*, Chen, P.*. Linking chromatin acylation mark-defined proteome and genome in living cells. Cell. 2023 Mar 2; 186(5):1066.
15. Jiang, Y., Huang, J., Tian, K., Zheng, H., Zhu, Y., Guo, T., Ji, X.*. Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture. Genome Biology. 2022 Nov 28;23(1):246.
16. Wang, H., Li, B., Zuo, L., Wang, B., Yan, Y., Tian, K., Zhou, R., Wang, C., Chen, X., Jiang, Y., Zheng, H., Qin, F., Zhang, B., Yu, Y., Liu, C., Xu, Y., Gao, J., Qi, Z., Deng, W., Ji, X.*, The transcriptional coactivator RUVBL2 regulates Pol II clustering with diverse transcription factors. Nature Communications. 2022 Sep 28;13(1):5703.
17. Zhou, R., Tian, K., Huang, J., Duan, W., Fu, H., Feng, Y., Wang, H., Jiang, Y., Li, Y., Wang, R., Hu, J., Ma, H., Qi, Z.*, Ji X.*, CTCF DNA binding domain undergoes dynamic and selective protein–protein interactions. iScience. 2022 Sep 16; 25(9).
18. Li, Y., Huang. J., Zhu J., Bao, L., Wang, H., Jiang, Y., Tian, K., Wang, R., Zheng, H., Duan, W., Lai, W., Yi, X., Zhu, Y., Guo, T., Ji X.*, Targeted protein degradation reveals Pol II heterogeneity and functional diversity. Molecular Cell. 2022 Sep 9: S1097-2765(22)00812-7.
19. Wang, C., Xu, Q., Zhang, X., Day, D.S., Abraham, B.J., Lun, K., Chen, L., Huang, J.*, Ji, X.*, BRD2 interconnects with BRD3 to facilitate Pol II transcription initiation and elongation to prime promoters for cell differentiation. Cell Mol Life Sci. 2022 Jun 4;79(6):338.
20. Cao, L., Luo, Y., Guo, X., Liu, S., Li, S., Li, J., Zhang, Z., Zhao, Y., Zhang, Q., Gao, F., Ji, X., Gao, X., Li, Y., You, F., SAFA facilitates chromatin opening of immune genes through interacting with anti-viral host RNAs. PLoS Pathog. 2022 Jun 3;18(6).
21. Xie, X., Gan, T., Rao, B., Zhang, W., Panchakshari, R.A., Yang, D., Ji, X., Cao, Y., Alt, F.W., Meng, F.L., Hu, J., C-terminal deletion-induced condensation sequesters AID from IgH targets in immunodeficiency. EMBO J. 2022 Jun 1;41(11).
22. Liu, Y., Ai, C., Gan, T., Wu, J., Jiang, Y., Liu, X., Lu, R., Gao, N., Li, Q., Ji, X., Hu, J. Transcription shapes DNA replication initiation to preserve genome integrity. Genome Biology. 2021 Jun 9;22(1):176.
23. Yang, B., Li, B., Jia, Li., Wang, X., Jiang, Y., Ji, X.*, Yang, P.*, 3D landscape of Hepatitis B virus interactions with human chromatins. Cell Discovery. 2020 Dec 29;6(1):95.
24. Jiang, Y., Huang, J., Lun, K., Li, B., Li, Y., Zheng, H., Li, Y., Zhou, R., Duan, W., Wang, C., Feng, Y., Yao, H., Li, C., Ji, X.*, Genome-wide Analyses of Chromatin Interactions After the Loss of Pol I, Pol II and Pol III. Genome Biology. 2020 Jul 2;21(1):158.
25. Zhang, H.*, Ji, X.*, Li, P.*, Liu, C.*, Lou, J.*, Wang, Z., Wen, W.*, Xiao, Y., Zhang, M.*, Zhu, X.*, Liquid-liquid Phase Separation in Biology: Mechanisms, Physiological Functions and Human Diseases. Science China Life Sciences. 2020 April 30.
26. Huang, J.*, Jiang, Y., Zheng, H., Ji, X.*, BAT Hi-C Maps Global Chromatin Interactions in An Efficient and Economical Way. Methods. 2020 Jan 1; 170: 38-47.
27. Ji, X., Dadon, D.B., Powell, B.E., Fan, Z.P., Borges-Rivera, D., Shachar, S., Weintraub, A.S., Hnisz, D., Pegoraro, G., Lee, T.I., et al. (2016). 3D Chromosome Regulatory Landscape of Human Pluripotent Cells. Cell Stem Cell. 18, 262-275.
28. Liu, X.S., Wu, H., Ji, X., Stelzer, Y., Wu, X., Czauderna, S., Shu, J., Dadon, D., Young, R.A., and Jaenisch, R. (2016). Editing DNA Methylation in the Mammalian Genome. Cell. 167, 233-247 e217.
29. Sigova, A.A., Abraham, B.J., Ji, X., Molinie, B., Hannett, N.M., Guo, Y.E., Jangi, M., Giallourakis, C.C., Sharp, P.A., and Young, R.A. (2015). TranscrIption factor trapping by RNA in gene regulatory elements. Science. 350, 978-981.
30. Ji, X., Dadon, D.B., Abraham, B.J., Lee, T.I., Jaenisch, R., Bradner, J.E., and Young, R.A. (2015). Chromatin proteomic profiling reveals novel proteins associated with histone-marked genomic regions. Proceedings of the National Academy of Sciences of the United States of America. 112, 3841-3846.
31. Fong, N., Kim, H., Zhou, Y., Ji, X., Qiu, J., Saldi, T., Diener, K., Jones, K., Fu, X.D., and Bentley, D.L. (2014). Pre-mRNA splicing is facilitated by an optimal RNA polymerase II elongation rate. Genes & Development. 28, 2663-2676.
32. Wang, Y., Jiang, L., Ji, X., Yang, B., Zhang, Y., and Fu, X.D. (2013). Hepatitis B viral RNA directly mediates down-regulation of the tumor suppressor microRNA miR-15a/miR-16-1 in hepatocytes. The Journal of biological chemistry. 288, 18484-18493.
33. Mo, S., Ji, X., and Fu, X.D. (2013). Unique role of SRSF2 in transcription activation and diverse functions of the SR and hnRNP proteins in gene expression regulation. Transcription. 4, 251-259.
34. Ji, X., Zhou, Y., Pandit, S., Huang, J., Li, H., Lin, C.Y., Xiao, R., Burge, C.B., and Fu, X.D. (2013). SR proteins collaborate with 7SK and promoter-associated nascent RNA to release paused polymerase. Cell. 153, 855-868.
35. Han, J., Ji, X., Wang, D., and Fu, X.D. (2011). Pre-mRNA splicing: where and when in the nucleus. Trends in Cell Biology. 21, 336-343.
36. Ji, X., and Fu, X.D. (2012). The mediator couples transcription and splicing. Molecular Cell. 45, 433-434.
37. Xue, Y., Zhou, Y., Wu, T., Zhu, T., Ji, X., Kwon, Y.S., Zhang, C., Yeo, G., Black, D.L., Sun, H., et al. (2009). Genome-wide analysis of PTB-RNA interactions reveals a strategy used by the general splicing repressor to modulate exon inclusion or skipping. Molecular Cell. 36, 996-1006.